"The field of Transient Receptor Potential (TRP) channels has gained momentum in recent years not only because of the award of 2021 Nobel Prize in Physiology or Medicine to David Julius and Ardem Patapoutian for their discoveries of receptors for temperature and touch, but also because of the growing appreciation of the diverse and important physiological and pathophysiological functions of this diverse family of cation channels. In the past decade, there have been important discoveries in the TRP field: resolution of the 3-D structures of major subfamilies using single particle cryogenic electron microscopy and X-ray crystallography, identification and development of selective agonists and antagonists for most TRP superfamilies, discovery of new human diseases associated with TRP gene mutations, as well as improved understanding of TRP channel function and regulation in various systems. These new discoveries are addressed in TRP-Mediated Signaling, a volume in the Methods in Signal Transduction book series"--